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contextual fear conditioning cfc paradigm  (Med Associates Inc)


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    Med Associates Inc contextual fear conditioning cfc paradigm
    a. Schematic representation of intracranial injection of the astrocyte membrane-specific adeno-associated virus (AAV5.GFAP.hM3D(Gq).mCherry) in the dHipp on PND 26. Beginning at PND 30, animals received AIE or H 2 O intermittently through PND 45. Following 26 day forced abstinence period, animals received saline or clozapine-N-oxide (CNO, i.p. 2 mg/kg) to activate astrocytes. After 30 minutes, animals were assessed in a contextual fear <t>conditioning</t> paradigm. Animals were placed in context A and subjected to 3-foot shocks across a 10-minute test period on day 1. On day 2 and 3, animals were placed in context A for 12 min with no foot shocks. b. Representative images of the dHipp, DAPI (blue), hM3DGq (magenta), GFAP (green), and colocalization of GFAP+hM3DGq confirming astrocyte specificity of AAV. c. Representative images of DeepLabCut output of labeled body parts on the x-y axis of the context box. d. Quantification of freezing behavior in the <t>CFC</t> task (H 2 O+SAL (#) vs. AIE+SAL ($); p = 0.029-0.085; H 2 O+CNO (&) vs. AIE+CNO (@); p = 0.062-0.574 ). e-f . Freeze/dart behavior quantification from sessions collapsed across foot shocks from Day 1 of the CFC. g-h. Freeze/dart behavior quantification from Days 2 and 3 of the CFC. Analysis: Repeated measures Two-way ANOVA, n = 12 animals/treatment group across 2 separate cohorts. ( p < 0.05* p < 0.001**)
    Contextual Fear Conditioning Cfc Paradigm, supplied by Med Associates Inc, used in various techniques. Bioz Stars score: 97/100, based on 76 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/contextual+fear+conditioning+cfc+paradigm/Contextual+Conditioning+Protocol+Package/bio_rxiv__2025__10__14__682478-119-10-18
    Average 97 stars, based on 76 article reviews
    contextual fear conditioning cfc paradigm - by Bioz Stars, 2026-09
    97/100 stars

    Images

    1) Product Images from "Adolescent Alcohol Exposure Disrupts Astrocyte-Synaptic Structural And Functional Coupling In The Male Dorsal Hippocampus"

    Article Title: Adolescent Alcohol Exposure Disrupts Astrocyte-Synaptic Structural And Functional Coupling In The Male Dorsal Hippocampus

    Journal: bioRxiv

    doi: 10.1101/2025.10.14.682478

    a. Schematic representation of intracranial injection of the astrocyte membrane-specific adeno-associated virus (AAV5.GFAP.hM3D(Gq).mCherry) in the dHipp on PND 26. Beginning at PND 30, animals received AIE or H 2 O intermittently through PND 45. Following 26 day forced abstinence period, animals received saline or clozapine-N-oxide (CNO, i.p. 2 mg/kg) to activate astrocytes. After 30 minutes, animals were assessed in a contextual fear conditioning paradigm. Animals were placed in context A and subjected to 3-foot shocks across a 10-minute test period on day 1. On day 2 and 3, animals were placed in context A for 12 min with no foot shocks. b. Representative images of the dHipp, DAPI (blue), hM3DGq (magenta), GFAP (green), and colocalization of GFAP+hM3DGq confirming astrocyte specificity of AAV. c. Representative images of DeepLabCut output of labeled body parts on the x-y axis of the context box. d. Quantification of freezing behavior in the CFC task (H 2 O+SAL (#) vs. AIE+SAL ($); p = 0.029-0.085; H 2 O+CNO (&) vs. AIE+CNO (@); p = 0.062-0.574 ). e-f . Freeze/dart behavior quantification from sessions collapsed across foot shocks from Day 1 of the CFC. g-h. Freeze/dart behavior quantification from Days 2 and 3 of the CFC. Analysis: Repeated measures Two-way ANOVA, n = 12 animals/treatment group across 2 separate cohorts. ( p < 0.05* p < 0.001**)
    Figure Legend Snippet: a. Schematic representation of intracranial injection of the astrocyte membrane-specific adeno-associated virus (AAV5.GFAP.hM3D(Gq).mCherry) in the dHipp on PND 26. Beginning at PND 30, animals received AIE or H 2 O intermittently through PND 45. Following 26 day forced abstinence period, animals received saline or clozapine-N-oxide (CNO, i.p. 2 mg/kg) to activate astrocytes. After 30 minutes, animals were assessed in a contextual fear conditioning paradigm. Animals were placed in context A and subjected to 3-foot shocks across a 10-minute test period on day 1. On day 2 and 3, animals were placed in context A for 12 min with no foot shocks. b. Representative images of the dHipp, DAPI (blue), hM3DGq (magenta), GFAP (green), and colocalization of GFAP+hM3DGq confirming astrocyte specificity of AAV. c. Representative images of DeepLabCut output of labeled body parts on the x-y axis of the context box. d. Quantification of freezing behavior in the CFC task (H 2 O+SAL (#) vs. AIE+SAL ($); p = 0.029-0.085; H 2 O+CNO (&) vs. AIE+CNO (@); p = 0.062-0.574 ). e-f . Freeze/dart behavior quantification from sessions collapsed across foot shocks from Day 1 of the CFC. g-h. Freeze/dart behavior quantification from Days 2 and 3 of the CFC. Analysis: Repeated measures Two-way ANOVA, n = 12 animals/treatment group across 2 separate cohorts. ( p < 0.05* p < 0.001**)

    Techniques Used: Injection, Membrane, Virus, Saline, Labeling

    Related Articles

    Injection:

    Article Title: Adolescent Alcohol Exposure Disrupts Astrocyte-Synaptic Structural And Functional Coupling In The Male Dorsal Hippocampus
    Article Snippet: Following the 26-day forced abstinence period, animals underwent a 3-day contextual fear conditioning (CFC) paradigm previously described using Med Associates operant chambers.

    Membrane:

    Article Title: Adolescent Alcohol Exposure Disrupts Astrocyte-Synaptic Structural And Functional Coupling In The Male Dorsal Hippocampus
    Article Snippet: Following the 26-day forced abstinence period, animals underwent a 3-day contextual fear conditioning (CFC) paradigm previously described using Med Associates operant chambers.

    Virus:

    Article Title: Adolescent Alcohol Exposure Disrupts Astrocyte-Synaptic Structural And Functional Coupling In The Male Dorsal Hippocampus
    Article Snippet: Following the 26-day forced abstinence period, animals underwent a 3-day contextual fear conditioning (CFC) paradigm previously described using Med Associates operant chambers.

    Saline:

    Article Title: Adolescent Alcohol Exposure Disrupts Astrocyte-Synaptic Structural And Functional Coupling In The Male Dorsal Hippocampus
    Article Snippet: Following the 26-day forced abstinence period, animals underwent a 3-day contextual fear conditioning (CFC) paradigm previously described using Med Associates operant chambers.

    Labeling:

    Article Title: Adolescent Alcohol Exposure Disrupts Astrocyte-Synaptic Structural And Functional Coupling In The Male Dorsal Hippocampus
    Article Snippet: Following the 26-day forced abstinence period, animals underwent a 3-day contextual fear conditioning (CFC) paradigm previously described using Med Associates operant chambers.



    Similar Products

    contextual fear conditioning cfc paradigm  (Med Associates Inc)
    97
    Med Associates Inc contextual fear conditioning cfc paradigm
    a. Schematic representation of intracranial injection of the astrocyte membrane-specific adeno-associated virus (AAV5.GFAP.hM3D(Gq).mCherry) in the dHipp on PND 26. Beginning at PND 30, animals received AIE or H 2 O intermittently through PND 45. Following 26 day forced abstinence period, animals received saline or clozapine-N-oxide (CNO, i.p. 2 mg/kg) to activate astrocytes. After 30 minutes, animals were assessed in a contextual fear <t>conditioning</t> paradigm. Animals were placed in context A and subjected to 3-foot shocks across a 10-minute test period on day 1. On day 2 and 3, animals were placed in context A for 12 min with no foot shocks. b. Representative images of the dHipp, DAPI (blue), hM3DGq (magenta), GFAP (green), and colocalization of GFAP+hM3DGq confirming astrocyte specificity of AAV. c. Representative images of DeepLabCut output of labeled body parts on the x-y axis of the context box. d. Quantification of freezing behavior in the <t>CFC</t> task (H 2 O+SAL (#) vs. AIE+SAL ($); p = 0.029-0.085; H 2 O+CNO (&) vs. AIE+CNO (@); p = 0.062-0.574 ). e-f . Freeze/dart behavior quantification from sessions collapsed across foot shocks from Day 1 of the CFC. g-h. Freeze/dart behavior quantification from Days 2 and 3 of the CFC. Analysis: Repeated measures Two-way ANOVA, n = 12 animals/treatment group across 2 separate cohorts. ( p < 0.05* p < 0.001**)
    Contextual Fear Conditioning Cfc Paradigm, supplied by Med Associates Inc, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/contextual+fear+conditioning+cfc+paradigm/Contextual+Conditioning+Protocol+Package/bio_rxiv__2025__10__14__682478-119-10-18
    Average 97 stars, based on 1 article reviews
    contextual fear conditioning cfc paradigm - by Bioz Stars, 2026-09
    97/100 stars
    		  Buy from Supplier

    Image Search Results


    a. Schematic representation of intracranial injection of the astrocyte membrane-specific adeno-associated virus (AAV5.GFAP.hM3D(Gq).mCherry) in the dHipp on PND 26. Beginning at PND 30, animals received AIE or H 2 O intermittently through PND 45. Following 26 day forced abstinence period, animals received saline or clozapine-N-oxide (CNO, i.p. 2 mg/kg) to activate astrocytes. After 30 minutes, animals were assessed in a contextual fear conditioning paradigm. Animals were placed in context A and subjected to 3-foot shocks across a 10-minute test period on day 1. On day 2 and 3, animals were placed in context A for 12 min with no foot shocks. b. Representative images of the dHipp, DAPI (blue), hM3DGq (magenta), GFAP (green), and colocalization of GFAP+hM3DGq confirming astrocyte specificity of AAV. c. Representative images of DeepLabCut output of labeled body parts on the x-y axis of the context box. d. Quantification of freezing behavior in the CFC task (H 2 O+SAL (#) vs. AIE+SAL ($); p = 0.029-0.085; H 2 O+CNO (&) vs. AIE+CNO (@); p = 0.062-0.574 ). e-f . Freeze/dart behavior quantification from sessions collapsed across foot shocks from Day 1 of the CFC. g-h. Freeze/dart behavior quantification from Days 2 and 3 of the CFC. Analysis: Repeated measures Two-way ANOVA, n = 12 animals/treatment group across 2 separate cohorts. ( p < 0.05* p < 0.001**)

    Journal: bioRxiv

    Article Title: Adolescent Alcohol Exposure Disrupts Astrocyte-Synaptic Structural And Functional Coupling In The Male Dorsal Hippocampus

    doi: 10.1101/2025.10.14.682478

    Figure Lengend Snippet: a. Schematic representation of intracranial injection of the astrocyte membrane-specific adeno-associated virus (AAV5.GFAP.hM3D(Gq).mCherry) in the dHipp on PND 26. Beginning at PND 30, animals received AIE or H 2 O intermittently through PND 45. Following 26 day forced abstinence period, animals received saline or clozapine-N-oxide (CNO, i.p. 2 mg/kg) to activate astrocytes. After 30 minutes, animals were assessed in a contextual fear conditioning paradigm. Animals were placed in context A and subjected to 3-foot shocks across a 10-minute test period on day 1. On day 2 and 3, animals were placed in context A for 12 min with no foot shocks. b. Representative images of the dHipp, DAPI (blue), hM3DGq (magenta), GFAP (green), and colocalization of GFAP+hM3DGq confirming astrocyte specificity of AAV. c. Representative images of DeepLabCut output of labeled body parts on the x-y axis of the context box. d. Quantification of freezing behavior in the CFC task (H 2 O+SAL (#) vs. AIE+SAL ($); p = 0.029-0.085; H 2 O+CNO (&) vs. AIE+CNO (@); p = 0.062-0.574 ). e-f . Freeze/dart behavior quantification from sessions collapsed across foot shocks from Day 1 of the CFC. g-h. Freeze/dart behavior quantification from Days 2 and 3 of the CFC. Analysis: Repeated measures Two-way ANOVA, n = 12 animals/treatment group across 2 separate cohorts. ( p < 0.05* p < 0.001**)

    Article Snippet: Following the 26-day forced abstinence period, animals underwent a 3-day contextual fear conditioning (CFC) paradigm previously described using Med Associates operant chambers.

    Techniques: Injection, Membrane, Virus, Saline, Labeling